Anti-ASK 1 Antibody

Raised in rabbit against human Apoptosis Signal-regulating Kinase 1 (ASK 1, also called MAP3K5), this unconjugated polyclonal IgG reagent from Antibodies.com is supplied as 100 µl of ready-to-use stock for western blot, immunohistochemistry, and ELISA workflows.

ASK 1 sits at the apex of stress-activated MAPK cascades: threonine and tyrosine phosphorylation of ASK 1 activates the MKK4/MKK7–JNK and MKK3/MKK6–p38 axes, driving transcriptional responses to reactive oxygen species, TNF-alpha, endoplasmic reticulum stress, and calcium overload. Researchers studying cardiac hypertrophy, neurodegeneration, ischemia-reperfusion injury, and cytokine-driven inflammation use anti-ASK 1 antibodies to track activation state, subcellular redistribution to mitochondria, and complex formation with partners such as thioredoxin and TRAF2.

The antibody binds the human target and is suitable for detection of endogenous ASK 1 in cell lysates and tissue sections, with HRP- or fluorophore-conjugated secondary antibodies easily paired in a single-use workflow. Aliquot the stock vial on receipt and refrigerate to limit freeze-thaw cycles between experiments.