Anti-ARL4 Antibody

This rabbit polyclonal antibody targets ARL4 (ADP-ribosylation factor-like 4), a small GTP-binding protein of the ARF/Arl family that participates in membrane trafficking, actin cytoskeleton remodeling, and Wnt/β-catenin signaling. The reagent is supplied by Antibodies.com as an unconjugated, liquid format at 100 µl per vial, raised in rabbit and affinity-purified for use as a primary antibody in research settings.

Validated applications include Western blot (WB) detection of endogenous ARL4 in cell lysates and immunohistochemistry (IHC) on formalin-fixed paraffin-embedded tissue sections. The antibody recognizes human ARL4 and has been used in studies of hepatocellular carcinoma, colorectal cancer, and neuronal development, where ARL4 acts as a downstream effector of Wnt signaling and contributes to invasive cell behavior and tumor progression.

For handling, aliquot upon first use and store at -20 °C in working volumes to limit freeze-thaw cycles; short-term storage at 4 °C is acceptable for up to two weeks. The unconjugated format allows flexible pairing with HRP-, fluorescent-, or biotin-labeled secondary reagents depending on assay design, supporting both chromogenic and immunofluorescence workflows in basic and translational research on small GTPases.