Anti-ARID5B Antibody

Raised in rabbits against the AT-rich interaction domain-containing transcription factor ARID5B, this unconjugated polyclonal IgG reagent from Antibodies.com is supplied as a 200 µl liquid sample sized for routine western blot workflows on human samples. The antibody recognizes an endogenous nuclear protein that functions as a transcriptional activator or repressor depending on promoter context, with characterized roles in hepatocyte gene expression, adipogenesis regulation, and inflammatory cytokine signaling through TNF-α pathway modulation. Researchers use ARID5B-targeting reagents to investigate B-lymphocyte lineage commitment and the genetic basis of childhood acute lymphoblastic leukemia susceptibility, where ARID5B risk variants have been identified through genome-wide association studies.

For western blot detection, the reagent is intended for lysates from human cell lines and primary tissue under denaturing, reducing conditions. The polyclonal format provides epitope coverage across multiple antigenic sites on the AT-rich interaction domain, supporting recognition in samples where post-translational modification or isoform variation might otherwise mask binding. Refrigerate on receipt, aliquot after first thaw to limit freeze-thaw cycles, and protect from light to preserve IgG activity across multiple experimental runs.