Anti-ARHGDIA Antibody

Manufactured by Antibodies.com, this rabbit polyclonal antibody targets ARHGDIA, a Rho GDP-dissociation inhibitor also known by the synonyms Rho GDI 1, Rho-GDI alpha, and GDIA1, which plays a central role in regulating Rho GTPase signaling and cytoskeletal dynamics. The reagent is supplied as an unconjugated IgG at a concentration of 1.0 mg/mL in a 50 µL vial, formulated in phosphate-buffered saline (without Mg²⁺ and Ca²⁺) at pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol as a cryoprotectant. It was raised against a recombinant human ARHGDIA immunogen and purified by affinity chromatography using that same immunogen, ensuring specificity for the endogenous total ARHGDIA protein. The antibody is validated for Western blot (WB) and immunocytochemistry/immunofluorescence (ICC/IF) applications, with reactivity confirmed across human, mouse, and rat samples, making it a versatile choice for comparative studies in cell biology and signal transduction research. For handling and storage, the product is shipped on cold packs at 4 °C; upon receipt it should be aliquoted and stored at –20 °C, with repeated freeze/thaw cycles avoided to preserve antibody integrity. Intended strictly for research use, this reagent is not approved for diagnostic or therapeutic purposes and is provided as part of the Antibodies.com Primary Antibodies catalogue for laboratory applications including the investigation of Rho-mediated pathways in cell migration, adhesion, and proliferation.