Anti-ARHGAP25 Antibody

ARHGAP25 (Rho GTPase Activating Protein 25) is a RhoGAP-family regulator that controls Rac1 GTPase activity in hematopoietic cells and is investigated for its role in leukocyte chemotaxis, phagocytosis, and inflammatory signaling. This unconjugated rabbit polyclonal antibody from Antibodies.com is raised against the ARHGAP25 target and is validated for western blot and immunohistochemistry workflows across human, mouse, and rat sample types.

Provided as 100 µl of polyclonal antiserum in liquid format, the reagent supports detection of endogenous ARHGAP25 expression in cell lysates and tissue sections. Polyclonal reactivity increases the likelihood of recognizing multiple epitopes on the target, which can be useful when studying dynamic regulation of Rac1-GTP loading during immune cell activation or cytoskeletal remodeling experiments.

Typical laboratory contexts include profiling RhoGAP expression in neutrophil and macrophage models, comparing ARHGAP25 levels in tumor versus matched normal tissue by IHC, and confirming CRISPR or siRNA knockdown efficiency on western blot. The unconjugated format allows researchers to pair the antibody with a custom HRP, fluorophore, or biotin secondary label of their choice.