Anti-ARF6 Antibody

ARF6 (ADP-ribosylation factor 6) is a small GTPase belonging to the ARF family, playing a central role in endosomal membrane trafficking, receptor recycling, and cortical actin remodeling at the plasma membrane. This unconjugated rabbit polyclonal antibody from Antibodies.com is raised against ARF6 and is supplied as 100 µl of liquid reagent validated for western blot, immunohistochemistry, and immunofluorescence workflows.

The antibody recognizes ARF6 across human, mouse, and rat samples, supporting comparative studies in cell lines and primary tissue. Common research applications include tracking of clathrin-independent endocytosis, phagocytic cup formation, and invadopodia biology in metastatic cancer models, where elevated ARF6-GTP levels have been linked to invasive phenotypes.

Researchers typically apply this reagent at recommended starting dilutions on cultured cells and frozen tissue sections, pairing it with fluorescent or HRP-conjugated secondary antibodies for multiplexed detection. Store the vial refrigerated between uses to preserve polyclonal titer across long-running studies of membrane trafficking dynamics.