Anti-ARF5 Antibody

Antibodies.com offers a rabbit polyclonal antibody raised against ARF5, validated for Western blot on human, mouse and rat samples. The unconjugated IgG reagent is supplied as a liquid in Phosphate Buffered Saline (pH 7.3) containing 50% glycerol and 0.02% sodium azide, and was generated using a recombinant fusion protein corresponding to amino acids 1–180 of human ARF5 (NP_001653.1). Affinity-purified antibody targets a protein of approximately 16 kDa and is recommended for use at a dilution range of 1:1,000 to 1:2,000 for Western blot. Concentration is lot specific. The product ships at 4°C and should be aliquoted and stored at -20°C upon delivery, with repeated freeze/thaw cycles avoided.

The supplied Western blot validation image shows extracts of various cell lines loaded at 25 µg per lane, probed with the antibody at 1:1,000 dilution and detected with a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody at 1:10,000 dilution in 3% non-fat dry milk in TBST, using an ECL Basic Kit with a 10-second exposure.