Anti-ARF3 Antibody

This rabbit polyclonal antibody from Antibodies.com targets ARF3 (ADP-ribosylation factor 3), a member of the ARF family of small GTP-binding proteins that regulate vesicular trafficking, membrane dynamics, and actin cytoskeleton remodeling at the Golgi and endosomal compartments. The unconjugated IgG reagent is supplied at 50 µl per vial and is validated for immunofluorescence (IF) applications on human samples, making it a practical tool for subcellular localization studies by confocal or fluorescence microscopy.

ARF3 participates in coat protein recruitment, cargo sorting, and the maintenance of organelle identity, and antibodies raised against it are commonly used to investigate Golgi architecture, brefeldin A-sensitive trafficking pathways, and the roles of Arf GTPases in cancer cell migration and neuronal signaling. As a polyclonal reagent, this antibody offers the advantage of recognizing multiple epitopes on the target, which can improve signal detection in tissues where ARF3 expression is low or where fixation conditions partially mask antigenic sites.

For optimal results, samples should be prepared according to standard IF protocols, including appropriate fixation, permeabilization, and blocking steps, with the antibody diluted to a working concentration determined empirically for each application. The unconjugated format allows flexible pairing with fluorophore-conjugated secondary antibodies, supporting multicolor imaging experiments alongside other trafficking or organelle markers.