Anti-ARF1 Antibody

The Anti-ARF1 Antibody from Antibodies.com is a rabbit polyclonal reagent raised against human ADP-ribosylation factor 1, a ~21 kDa small GTPase that regulates vesicular trafficking, COPI coat assembly at the Golgi, and ARF-dependent activation of phospholipase D. Provided as an unconjugated, liquid preparation in a 100 µl vial, the antibody is validated for Western blot and immunohistochemistry workflows and is reactive against human samples, supporting cell biology labs studying membrane traffic between the endoplasmic reticulum and the Golgi apparatus, vesicle biogenesis, and the broader Ras-related GTPase signaling network.

Because ARF1 sits at a nexus of coat recruitment, lipid metabolism, and cargo sorting, this reagent is applied in studies of secretory pathway organization, endocytosis, actin remodeling at the cell periphery, and pathogen-driven manipulation of host trafficking machinery. It is also used in oncology contexts where ARF1 expression has been investigated in breast, lung, and colorectal tumor cell behavior. Researchers using the antibody for Western blot resolve ARF1 in the ~20-25 kDa region of an SDS-PAGE gel, while IHC applications enable visualization of ARF1 distribution across fixed tissue sections.

For storage, the antibody should be kept refrigerated and protected from repeated freeze-thaw cycles to preserve polyclonal reactivity over the working shelf life. Aliquot on first use if frequent sampling is anticipated. The unconjugated format allows the end user to pair the primary with a custom-labeled secondary antibody of their choice, providing flexibility across fluorescence, chromogenic, and chemiluminescent detection platforms. Each vial ships ready for dilution into standard assay buffers at typical working concentrations recommended for rabbit polyclonal primaries.