Anti-AR (phospho S213) Antibody

A rabbit polyclonal IgG reagent directed against a phosphorylated epitope of the human androgen receptor, recognizing the serine 213 (S213) phospho-site. Produced by Antibodies.com as a primary antibody, the unconjugated reagent is supplied in a 50 µl volume and is validated for western blot (WB) and immunofluorescence (IF) applications on human samples.

The androgen receptor (AR) is a ligand-activated nuclear transcription factor central to androgen signaling and a heavily studied target in prostate cancer research. Phosphorylation at Ser213 has been described in published receptor studies as one of several post-translational modifications that influence AR stability, subcellular localization, and transcriptional activity. Tracking the phospho-S213 state alongside total AR levels is therefore useful for comparing activated versus quiescent receptor states in cell-based models.

For western blot, the reagent is expected to detect an approximately 110 kDa band corresponding to full-length AR in human cell lysates. In immunofluorescence, predicted staining is predominantly nuclear in AR-positive lines commonly used in prostate cancer research, with appropriate negative controls recommended to confirm phospho-specificity.

Storage at -20 °C in working aliquots is advised to preserve phospho-epitope integrity and to limit freeze-thaw cycles. Host species is rabbit, clonality is polyclonal, isotype IgG, and the reagent carries no attached fluorophore or enzyme, leaving secondary detection chemistry open to the end user. Reactivity is reported for human androgen receptor.