Anti-AR-β2 (phospho-S346) Antibody

Phosphorylation at serine 346 of the β2-adrenergic receptor (AR-β2) acts as a regulatory switch controlling receptor desensitization, internalization, and downstream G-protein signaling cascades. This rabbit polyclonal affinity-purified antibody, distributed by Antibodies.com within their Primary Antibodies range, recognizes the phospho-S346 epitope rather than total receptor protein, making it a useful reagent for western blot studies of agonist-driven receptor regulation in both transfected cells and native tissues. The product is supplied as 100 µl of a 1 mg/ml stock in phosphate buffered saline (pH 7.2) containing 0.05% sodium azide as a preservative.

Validation covers western blot, and the antibody cross-reacts with human, mouse, and rat AR-β2, supporting comparative work across common laboratory species. In practice, researchers apply it to monitor GRK- and PKA-dependent receptor phosphorylation after stimulation with β-agonists such as isoproterenol or albuterol, to characterize biased agonism at the receptor, and to profile how β-blockers alter basal phosphorylation status in airway smooth muscle, cardiac tissue, and heterologous expression systems. Because the reagent is unconjugated, it pairs with any HRP- or fluorophore-labelled anti-rabbit secondary reagent selected by the end user. Aliquot upon first use, keep working stocks refrigerated, and avoid repeated freeze-thaw cycles on the diluted preparation; long-term archives are held at -20 °C or colder, consistent with standard handling of phospho-specific polyclonal preparations.