Anti-APP Antibody

Built on the polyclonal backbone, this Anti-APP reagent from Antibodies.com detects epitopes distributed across the APP extracellular region, including the KPI-containing isoforms APP751 and APP770 alongside the neuron-enriched APP695 variant. Researchers probing synaptic fractions or Tris-soluble lysates benefit from the antibody's recognition of both immature and mature glycosylated species, visible as a characteristic doublet near 100-130 kDa on western blot. A working dilution of 1:500 to 1:2000 in 5% non-fat milk/TBST is generally appropriate for PVDF membranes, while immunocytochemistry on fixed neuronal cultures is typically performed at 1:100 to 1:500 in blocking buffer containing 1% BSA and 0.1% Triton X-100. The unconjugated IgG format allows pairing with HRP-conjugated secondary antibodies for chemiluminescent readout or with Alexa Fluor-labeled secondaries for fluorescence microscopy. Because APP gene dosage influences amyloid load, notably in trisomy 21 models, this reagent is frequently cited in studies comparing wild-type and Down syndrome-derived neural tissue. Short-term use within two weeks is acceptable at 4 °C once aliquoted, but long-term stability is best preserved at -20 °C in working volumes to minimize freeze-thaw cycles.