Anti-APP Antibody

Raised in rabbits and validated for Western blot detection of endogenous APP protein in human and mouse samples. The unconjugated IgG polyclonal reagent from Antibodies.com is supplied as a 200 µl liquid aliquot and binds amyloid precursor protein, a type I transmembrane glycoprotein implicated in neuronal adhesion, synaptic plasticity, and the amyloidogenic cleavage pathway associated with Alzheimer's disease. APP migrates as a cluster of bands between approximately 100 and 130 kDa on SDS-PAGE, with additional lower-molecular-weight species corresponding to proteolytic fragments such as soluble APP and amyloid-beta peptide; the neuronal isoform APP695 predominates in brain tissue while APP751 and APP770 are more broadly expressed. Aliquot upon receipt and store at -20°C to limit freeze-thaw damage, and use the reagent with standard chemiluminescent Western blot workflows on PVDF or nitrocellulose membranes using HRP-conjugated secondary antibodies. Researchers commonly deploy this reagent to monitor full-length APP expression and processing in transfected cell lines, primary neuron cultures, and brain tissue lysates, supporting studies of trafficking, secretase activity, and disease-associated proteolysis.