Anti-ApoF Antibody

ApoF (Apolipoprotein F) is a sialoglycoprotein component of circulating high-density lipoprotein particles, where it acts as a lipid transfer inhibitor and influences plasma cholesterol distribution. This unconjugated rabbit polyclonal reagent from Antibodies.com is raised against the human ApoF target and supports immunoblotting and ELISA workflows in cardiovascular and metabolic disease research. Each vial contains 100 µl of polyclonal antiserum, supplied as a research use only reagent suitable for probing cell or tissue lysates by western blot and for quantifying ApoF in conditioned media, lipoprotein fractions, and other samples by ELISA-based detection. Because the antibody is delivered without a fluorophore or enzyme label, individual laboratories can pair it with HRP, biotin, alkaline phosphatase, or bead-based detection chemistries matched to a chosen experimental design. The polyclonal host response enables recognition of multiple epitopes across the ApoF primary sequence, which increases the likelihood of productive signal whether the antigen is presented in native or denatured form. For long-term storage, aliquot the antiserum and freeze at -20 degrees C, protect from light, and limit freeze-thaw cycles to preserve titer. Investigators studying hepatic lipoprotein assembly, HDL remodeling, cholesterol homeostasis, or apolipoprotein-mediated lipid exchange in human cell culture models and primary samples will find this reagent useful for both qualitative and quantitative readouts, and conjugation flexibility makes it straightforward to adapt the antibody to established in-house immunoassay protocols.