Anti-APOE Antibody

Antibodies.com presents a rabbit polyclonal antibody to Apolipoprotein E (APOE), supplied as an unconjugated IgG reagent validated for Western blot, immunohistochemistry, and immunocytochemistry/immunofluorescence (ICC/IF) applications. The antibody was raised against a recombinant fusion protein containing a sequence corresponding to amino acids 41–307 of human APOE (NP_000032.1) and reacts with Human, Mouse, and Rat samples.

The reagent is affinity-purified and provided as a liquid in Phosphate Buffered Saline (pH 7.3) containing 50% glycerol and 0.05% Proclin 300; the target protein has an apparent molecular weight of 35 kDa. Recommended working dilutions are 1:100–1:500 for Western blot, 1:50–1:200 for IHC, and 1:50–1:200 for ICC/IF. The antibody is shipped at 4 °C and should be aliquoted and stored at −20 °C, with freeze/thaw cycles avoided.

Supporting data include a Western blot of SH-SY5Y cell lysates at 1:500 dilution, plus immunohistochemistry images of paraffin-embedded human placenta, rat heart, and rat kidney sections each stained at 1:50.