Anti-APEH Antibody

This unconjugated polyclonal antibody from Antibodies.com is raised in rabbit against APEH (Acyl-peptide hydrolase, also known as AARE, acylamino-acid-releasing enzyme), a cytosolic serine peptidase that strips N-terminal acyl blocking groups from peptides to expose free N-termini. The reagent is supplied at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol as a stabiliser, packaged as a 100 µl vial.

Validated for Western blot and immunohistochemistry workflows, the antibody reacts with human, mouse, and rat samples, supporting cross-species analysis of APEH expression in cell lysates and fixed tissue sections. APEH is a serine protease tied to protein turnover and the ubiquitin-proteasome system, so this reagent aids studies of peptide metabolism, N-end rule pathways, and oxidative-stress responses in vitro and in vivo.

On Western blots, APEH typically migrates near 81 kDa by SDS-PAGE and is detected with HRP- or fluorophore-conjugated secondary reagents. The polyclonal format increases tolerance for minor sequence variation between species, useful when comparing rodent and human samples on the same membrane. The unconjugated format allows pairing with custom labels. Store refrigerated and protect from light to preserve activity.