Anti-APAF1 Antibody

Raised in rabbit against human APAF1 (Apoptotic Protease Activating Factor 1), this unconjugated polyclonal reagent from Antibodies.com supports research into the intrinsic mitochondrial apoptosis pathway, where APAF1 oligomerizes with cytochrome c and procaspase-9 to assemble the apoptosome and trigger the caspase cascade that drives programmed cell death.

Validated for Western blot and immunohistochemistry on human, mouse, and rat samples, the antibody enables detection of endogenous APAF1 in cell lysates and in paraffin-embedded or cryosectioned tissues. The reagent is supplied at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide at approximately pH 7.3, and is packaged as 100 µl that can be aliquoted and stored refrigerated between uses. Protect from light during handling to preserve the unconjugated immunoglobulin.

Investigators studying tumor suppression, neurodegeneration, ischemic injury, or developmental remodeling frequently rely on APAF1 detection to monitor apoptotic responsiveness across experimental systems.