Anti-Apaf-1-ALT Antibody

Rabbit polyclonal antibody targeting the Apaf-1-ALT splice variant of apoptotic protease-activating factor 1, supplied by Antibodies.com as an unconjugated reagent for apoptosis research. Apaf-1 is the cytosolic scaffold protein that oligomerizes into the apoptosome once cytochrome c is released from mitochondria, where it recruits and activates caspase-9 to launch the intrinsic cell death cascade. The Apaf-1-ALT isoform arises from alternative transcript processing and is of interest to groups characterizing how different Apaf-1 splice forms influence apoptosome assembly and programmed cell death signaling.

This primary antibody is raised in rabbit and validated for Western blot, immunohistochemistry, and immunofluorescence workflows on human samples, with ELISA listed among its supported applications. The polyclonal format offers recognition of multiple epitopes on the target, which is helpful when probing expression differences between full-length Apaf-1 and the alternative transcript. Provided as 100 µl of unconjugated antiserum in liquid format, the reagent allows flexible choice of secondary antibody for chromogenic, fluorescent, or chemiluminescent detection systems commonly used in cancer biology and mitochondrial pathway studies.

Short-term storage at 4 °C is appropriate, and aliquoting is recommended for longer term use to limit freeze-thaw cycles. Laboratories focused on cytochrome c-dependent apoptosis, intrinsic pathway regulation, or characterization of Apaf-1 isoform expression will find this reagent particularly useful given the limited availability of antibodies that distinguish the ALT variant from canonical Apaf-1.