Anti-AP-2 Antibody

AP-2 (Adaptor Protein Complex 2) is a heterotetrameric clathrin adaptor that drives cargo selection and vesicle budding at the plasma membrane. This rabbit polyclonal reagent from Antibodies.com is raised against the AP-2 target and validated for Western blot and immunohistochemistry workflows across human, mouse, and rat samples. Supplied unconjugated in a 100 µl volume, the antibody fits standard immunoblotting and tissue-section protocols without requiring stripping or pre-adsorption steps for routine reactivity. Researchers studying clathrin-mediated endocytosis, receptor internalization (such as transferrin and LDL receptor trafficking), and synaptic vesicle recycling commonly apply AP-2 antibodies to map adaptor localization, monitor complex assembly, or follow perturbations after RNAi or small-molecule inhibition. The polyclonal format increases the likelihood of recognizing multiple epitopes of the adaptor complex, an advantage when probing denatured lysates as well as formaldehyde-fixed sections. For handling, aliquot the reagent upon arrival and freeze for long-term storage; protect from light and avoid repeated freeze-thaw cycles to preserve binding activity.