Anti-ANP32D Antibody

The target ANP32D belongs to the acidic nuclear phosphoprotein 32 (ANP32) family, a group of regulators implicated in nuclear processes including chromatin-associated functions and protein phosphatase regulation. This unconjugated rabbit polyclonal reagent from Antibodies.com is supplied at 1 mg/ml in phosphate buffered saline (pH ~7.2) with 0.05% sodium azide as preservative, and is validated for Western blot detection of the endogenous protein in human, mouse, and rat samples.

The antibody is generated in rabbit and purified to yield an IgG fraction appropriate for immunoblotting of cell and tissue lysates. Researchers studying ANP32 family biology, paralog-specific expression, or comparative studies across species will find the cross-reactivity profile useful, supporting work in human, mouse, and rat cell lines and primary tissues.

For Western blot applications, the antibody is typically applied at working concentrations determined empirically by the user, with detection dependent on the specific gel conditions, transfer setup, and chemiluminescent substrate used. The 100 µl pack size provides ample volume for multiple blotting experiments. Store refrigerated; for long-term storage, aliquot to limit freeze-thaw cycles. Sodium azide is included as a preservative, so the reagent is not appropriate for live cell staining without prior dialysis or filtration.

This reagent is intended for research use only and is not validated for diagnostic or therapeutic applications. Users should optimize dilution, blocking conditions, and secondary antibody selection to match their specific substrate and detection system.