Anti-Annexin A5 Antibody

Sourced through Antibodies.com, this polyclonal reagent is a rabbit-derived IgG preparation raised against recombinant human Annexin A5 protein and affinity-purified by caprylic acid–ammonium sulfate precipitation. Supplied at 1.0 mg/mL in a stabilizer buffer containing 50% glycerol, 0.01 M PBS (pH 7.4) and 0.03% Proclin 300, each pack contains 100 µg of unconjugated antibody intended for western blot and immunohistochemistry workflows on human samples. The antibody recognizes endogenous total Annexin A5, with validation data showing a predicted and observed band size of approximately 35.5 kDa in EC109 and 293T whole-cell lysates at 2 µg/mL primary dilution, followed by a goat anti-rabbit IgG secondary at 1/15000. The product is shipped at 4 °C and should be aliquoted upon arrival for long-term storage at −20 °C, with repeated freeze/thaw cycles avoided to preserve activity. As a research-use-only reagent, it is well suited to laboratories studying annexin-mediated membrane binding, phosphatidylserine exposure and apoptosis-related signaling pathways in human cell and tissue models.