Anti-Annexin 2 Antibody

Raised in rabbit against the calcium-dependent phospholipid-binding protein Annexin 2 (ANXA2), this unconjugated polyclonal reagent from Antibodies.com is supplied as 100 µl of a 1 mg/ml stock in PBS with 0.1% sodium azide and 50% glycerol as a stabilizing cryoprotectant. The antibody cross-reacts with human, mouse, and rat samples, supporting comparative work across common laboratory models and translating between cell-line, primary-cell, and tissue-based experiments.

Validated applications include western blot, immunohistochemistry, immunofluorescence, immunoprecipitation, and RNA immunoprecipitation, covering uses that range from quantification of total Annexin 2 expression in whole-cell lysates and fixed tissue sections to affinity capture of the endogenous protein or its associated RNA transcripts from chromatin and ribonucleoprotein complexes. The unconjugated format allows users to pair the reagent with their own choice of secondary antibody or label, enabling chromogenic, fluorescent, or affinity-based detection on standard microscopy, blotting, and pull-down workflows.

Annexin 2 acts as a multifunctional scaffold at the plasma membrane and in the cytoplasm, participating in actin cytoskeleton remodeling, vesicle trafficking, exocytosis, and the cell-surface fibrinolytic system where it serves as a co-receptor for tissue plasminogen activator and plasminogen, and it has been implicated in viral entry pathways, which is why it is widely investigated in cancer, vascular biology, and infectious-disease research. The glycerol-containing formulation can be stored at -20 °C with minimal freeze-thaw cycles to preserve activity, while working aliquots may be refrigerated at 4 °C and the antibody is intended for research use only, not for diagnostic or therapeutic procedures.