Anti-ANKRD44 Antibody

Produced as a rabbit polyclonal reagent and unconjugated IgG preparation, this Antibodies.com antibody recognizes ANKRD44 (Ankyrin Repeat Domain 44), a scaffolding protein linked to T-cell receptor signaling and immune-cell function research. The antibody is supplied in a 200 µl vial and is validated for Western blot (WB) detection in human samples, supporting studies of lymphocyte signaling pathways and ankyrin-repeat-containing proteins investigated through immunoblotting.

As a primary antibody, the polyclonal format offers broader epitope coverage across ANKRD44 than a monoclonal reagent, helping to detect multiple regions of the target. Researchers can pair it with standard anti-rabbit IgG secondary reagents (HRP, fluorophore, or biotin conjugates) for chemiluminescent, fluorescent, or affinity-based detection workflows. The unconjugated format provides flexibility to match the experimental readout, whether film-based ECL, near-infrared imaging, or fluorescence-based scanning platforms.

Typical uses include confirmation of ANKRD44 expression in human cell lines and primary T-cell lysates, characterization of CRISPR knockout or knockdown phenotypes, and validation of transcriptomic findings at the protein level. Because the antibody is reactive with human ANKRD44, it complements expression and functional studies that link ankyrin-repeat scaffolding proteins to immune regulation and cytoskeletal organization. Store refrigerated and protect from light to preserve reagent activity.