Anti-ANKRD30A Antibody

Offered by Antibodies.com, this rabbit polyclonal reagent targets ANKRD30A (Ankyrin repeat domain-containing protein 30A), a 152 kDa antigen also known as serologically defined breast cancer antigen NY-BR-1. Supplied as a 50 µl aliquot at 1 mg/ml in an unconjugated, affinity-purified format, the antibody was raised in rabbit and purified from rabbit serum by antigen affinity chromatography using the immunizing peptide. The IgG isotype makes it compatible with a broad range of secondary detection reagents, and the product is validated for Western blotting, immunohistochemistry, and ELISA applications on human samples. Recommended working dilutions reported on the supplier page include WB at 1:500–1:1000 and ELISA starting around 1:100, with the antibody expected to detect endogenous ANKRD30A in cell and tissue lysates. For routine use, vials should be stored at 2–8 °C for short-term handling, while continuous use and long-term storage are best maintained at –20 °C in small aliquots to limit freeze–thaw cycles; the buffer contains 0.42% potassium phosphate, 0.87% sodium chloride (pH 7.3), 30% glycerol, and 0.01% sodium azide as a preservative. Researchers working on breast cancer biomarkers, ankyrin-repeat proteins, or tumor-associated antigens will find this polyclonal antibody a reliable tool for both qualitative and semi-quantitative studies, with the unconjugated format allowing flexible labelling with HRP, biotin, or fluorophore conjugates of choice. The reagent is intended for research use only and is shipped under standard cold-chain conditions to preserve activity.