Anti-ANGPT1 Antibody

This rabbit polyclonal IgG reagent from Antibodies.com is supplied as 100 µL of unconjugated liquid antiserum and was raised for selective detection of Angiopoietin-1 (ANGPT1) on Western blot membranes. The antibody specifically recognizes the secreted glycoprotein ligand of the TIE2 receptor tyrosine kinase, which is produced by pericytes and vascular smooth muscle cells and guides blood vessel maturation, endothelial cell quiescence, and postnatal angiogenic remodeling. Reactivity has been confirmed on human and mouse samples, supporting translational vascular biology studies, tumor angiogenesis screens, and cardiovascular disease models that compare ANGPT1 expression across species. The unconjugated format leaves the reporter choice to the laboratory, so the primary antibody can be paired with HRP, alkaline phosphatase, or fluorophore-labelled anti-rabbit IgG secondary reagents for chemiluminescent or fluorescent detection after SDS-PAGE separation of reduced whole-cell or tissue lysates. Angiopoietin-1 resolves near its expected glycosylated molecular weight on the blot, and the reagent is intended for research use only in investigations of the angiopoietin-TIE signaling axis, pericyte-endothelial crosstalk, and vascular stabilization. Storage conditions should follow the manufacturer's recommendation of aliquoting on arrival and avoiding repeated freeze-thaw cycles to preserve polyclonal activity.