Anti-ANAPC4 Antibody

Raised in rabbit against anaphase-promoting complex subunit 4 (APC4), this polyclonal antibody from Antibodies.com recognizes a core scaffolding component of the APC/C E3 ubiquitin ligase that drives mitotic exit and the metaphase-to-anaphase transition by targeting securin and cyclin B for proteasomal degradation.

Validated for Western blot and immunohistochemistry on Human and Mouse samples, the unconjugated reagent is supplied as 100 µl of antiserum and supports studies of chromosome segregation, spindle assembly checkpoint control, and cell-cycle progression in normal and transformed cells. Researchers use it to examine APC/C subunit composition, monitor ubiquitin-dependent proteolysis during mitosis, and probe how ANAPC4 expression changes in cancer cell lines and in tissue microarrays of proliferating versus quiescent cell populations.

Working dilutions, recommended buffers, and lot-specific validation data are provided on the product datasheet, and the reagent should be stored frozen in small aliquots to limit freeze-thaw cycles. For research use only.