Anti-Amyloid-β Antibody

Aim research at amyloid-β plaques and processing intermediates with this rabbit polyclonal reagent from Antibodies.com, supplied as 100 µl of unconjugated antiserum suitable for Western blot, immunohistochemistry, immunofluorescence, and ELISA workflows. The polyclonal clonality broadens epitope coverage across the Aβ peptide, helping distinguish full-length APP from its cleaved fragments in cell- and tissue-based assays.

Validated reactivity spans human, mouse, and rat samples, making this antibody a practical choice for Alzheimer's disease model work using transgenic mouse lines, postmortem human brain sections, and rodent primary neuronal cultures. Common applications include visualizing extracellular plaque deposits in fixed cortical and hippocampal tissue, tracking intracellular Aβ accumulation by immunofluorescence, and probing APP cleavage products on Western blot.

For immunohistochemistry on dense amyloid deposits, formic acid antigen retrieval is commonly used to unmask buried epitopes, while standard aldehyde fixation generally preserves immunofluorescence signal in cell culture work.