Anti-Amylin (H28) Antibody

The amylin peptide, also known as islet amyloid polypeptide (IAPP), is a 37-residue hormone co-secreted with insulin from pancreatic beta cells. It contributes to glycemic regulation and satiety signaling, and its aggregation into amyloid deposits in the islets of Langerhans is a hallmark of type 2 diabetes. This rabbit polyclonal reagent from Antibodies.com, clone designation H28, was raised against amylin and enables selective detection of the endogenous peptide in research samples.

Validated for Western blot, immunohistochemistry, and immunofluorescence, the antibody has been characterized for reactivity with human, mouse, and rat specimens. Its unconjugated format leaves primary amines available for downstream conjugation, so the antibody can be paired with HRP-, biotin-, Alexa Fluor-, or other anti-rabbit secondary reagents chosen by the researcher, supporting chromogenic, fluorescent, or tyramide signal amplification workflows.

Each vial is supplied at 1 mg/ml in phosphate buffered saline (PBS) with 0.05% sodium azide at approximately pH 7.2, in a 50 µl pack size that supports dozens of immunoblots or many tens of IHC slides depending on dilution. The sodium azide acts as a preservative; for direct conjugation or live-cell work, the azide should be removed by dialysis or buffer exchange prior to use.

Typical research applications include characterizing amyloid plaque composition in diabetic pancreas tissue, mapping co-localization with insulin in islet beta cells, profiling IAPP expression in central and peripheral neurons where amylin acts as a neuromodulator, and tracking aggregation-prone species in cellular and animal models of metabolic disease.