Anti-AMPKa1 (Ab-487)Antibody Antibody

Offered through Antibodies.com, this research-grade reagent is a rabbit polyclonal antibody directed against AMPKα1 at residue 487 (Ab-487), designed to detect the endogenous level of total AMPKα1 protein. The antibody was raised against a synthetic peptide corresponding to amino acids 485–489 (S-G-S-V-S) of the human AMPKα1 sequence and was subsequently purified by affinity chromatography using the epitope-specific peptide. It is supplied at 1.0 mg/mL in 50 µl of phosphate-buffered saline (pH 7.4, without Mg²⁺ and Ca²⁺), containing 150 mM sodium chloride, 0.02% sodium azide, and 50% glycerol as a cryoprotectant. The host species is rabbit, the clonality is polyclonal, and the reagent is provided unconjugated, giving researchers flexibility for downstream labelling in Western blot (WB) and immunohistochemistry (IHC) workflows. Reactivity has been validated for human samples, making the antibody well suited for studies in cell lines and tissue specimens of human origin. Recommended working dilutions fall in the range of 1:500–1:1000 for Western blot and 1:50–1:100 for immunohistochemistry, supporting robust signal detection across both blotting and chromogenic tissue-staining protocols. The product is intended for research use only and is not approved for diagnostic or therapeutic applications, so it should be handled using standard laboratory precautions and stored according to the manufacturer's recommendations to preserve reagent stability across multiple experiments.