Anti-AMPK gamma-2 Antibody

This affinity-purified reagent from Antibodies.com is a rabbit polyclonal antibody directed against the gamma-2 regulatory subunit of AMP-activated protein kinase (AMPK gamma-2, PRKAG2), raised against a recombinant protein fragment corresponding to amino acids 1–288 of the human target. The antibody is supplied at a volume of 100 µl in 0.1 M Tris-buffered saline (pH 7.0) containing 10% glycerol, with 0.01% thimerosal added as a preservative, and is presented in an unconjugated format suitable for direct labelling by the user. Validation data on the supplier page demonstrate clean Western blot and immunocytochemistry/immunofluorescence signals, with a recommended working dilution of 1:200 in paraformaldehyde-fixed HeLa cells, and the reagent is documented for use in WB and ICC/IF applications on human samples. As a polyclonal raised in rabbit, the antibody is purified by antigen-affinity chromatography to reduce background and is shipped at 4 °C; upon receipt it should be aliquoted and stored at –20 °C, with repeated freeze/thaw cycles avoided to preserve activity. Researchers evaluating the metabolic role of AMPK in cardiac and skeletal muscle, where the gamma-2 isoform predominates, will find this Antibodies.com reagent well suited to detecting endogenous PRKAG2 in cultured human cells, and its unconjugated format allows flexible pairing with secondary reporters of choice. The product is intended for research use only and is not licensed for diagnostic or therapeutic applications.