Anti-AMPK beta 1 Antibody

PRKAB1 Antibody for AMPK Signaling Research

This Mouse monoclonal antibody from Antibodies.com targets AMPK beta 1 (PRKAB1), the regulatory non-catalytic subunit of the heterotrimeric AMP-activated protein kinase complex. AMPK functions as a cellular energy sensor that is activated by rising AMP and falling ATP levels, and the beta 1 isoform contributes to glycogen binding, subcellular localization of the kinase, and stabilization of the alpha and gamma subunits within the holoenzyme.

Supplied as an unconjugated reagent in a 100 µl volume, the antibody is validated for Western blot (WB), immunocytochemistry (ICC), immunoprecipitation (IP), and immunohistochemistry (IHC). Reactivity has been confirmed in Human, Mouse, Rat, and Monkey samples, making it a practical choice for cross-species studies of AMPK signaling in metabolic disease research, cancer biology, exercise physiology, and hepatocyte or skeletal muscle investigations.

Typical uses include probing AMPK subunit composition in cell lysates, confirming beta 1 isoform expression across tissues, and immunoprecipitating endogenous complexes for downstream kinase activity assays or interactome analyses. For short-term use, refrigerate and protect from light; for extended storage, freeze at -20°C in working aliquots and limit freeze-thaw cycles to preserve antibody performance over the shelf life of the reagent.