Anti-AMPK alpha 2 Antibody

This rabbit polyclonal reagent from Antibodies.com targets AMPK alpha 2, the catalytic alpha-2 subunit of AMP-activated protein kinase encoded by the PRKAA2 gene. The unconjugated antibody is supplied at 100 µL per vial and is validated for western blot, immunohistochemistry, and immunofluorescence workflows on human samples. AMPK alpha 2 is broadly expressed in skeletal muscle, cardiac tissue, liver, and pancreatic islets, where it monitors cellular AMP/ATP balance and phosphorylates substrates controlling glucose uptake, fatty acid oxidation, mitochondrial biogenesis, and autophagy. Researchers commonly deploy this reagent to assess phosphorylation-dependent activation states, including the activating Thr172 site, and to compare alpha-2 versus alpha-1 isoform contributions in metabolic and cell signaling studies. The polyclonal format broadens epitope coverage across the target, which is useful when probing denatured whole-cell lysates or fixed tissue sections where a single monoclonal epitope may be masked. Storage at -20 °C in small aliquots is recommended to limit freeze-thaw cycles and preserve binding activity across repeated experiments.