Anti-AMPKβ1 (V175) Antibody

This rabbit polyclonal antibody from Antibodies.com recognizes AMPKα1, the catalytic α1 subunit of AMP-activated protein kinase (AMPK), a central cellular energy sensor activated when intracellular AMP:ATP ratios rise. AMPKα1 is the dominant catalytic isoform in many non-hepatic tissues, including skeletal muscle, cardiomyocytes, and neurons, where it phosphorylates downstream substrates such as ACC, ULK1, and TSC2 to coordinate metabolic adaptation under energy stress. The immunogen was a synthetic peptide corresponding to a region of human AMPKα1 that includes residue V175, and the reagent is supplied as 100 µl at 1 mg/ml in phosphate-buffered saline (PBS) with 0.05% sodium azide as a preservative, at approximately pH 7.2. It is validated for Western blot (WB) and immunohistochemistry (IHC) and cross-reacts with the mouse and rat orthologs alongside the human target, supporting comparative work across the three species most commonly used in metabolic and cell signaling research. Aliquots should be stored at -20°C for long-term use, with working stocks kept at 4°C to limit freeze-thaw cycles; the azide preservative permits brief handling at room temperature during routine bench work, though the solution should be protected from light. The unconjugated format gives researchers freedom to conjugate the antibody to HRP, fluorophores, or biotin for custom detection workflows in cell culture lysates, tissue cryosections, or other in vitro samples. As a polyclonal reagent, the antibody recognizes multiple epitopes on AMPKα1, often producing stronger signals than monoclonal alternatives when probing novel lysates or primary tissue for AMPK pathway activity.