Anti-AMPKβ1 (1A7) Antibody

Manufactured by Antibodies.com, this Mouse monoclonal antibody (clone 1A7) targets the regulatory subunit beta-1 of AMP-activated protein kinase (AMPKβ1), also known by the gene symbol PRKAB1. The reagent is supplied at a 50 µl pack size as a purified IgG isotype preparation in an unconjugated format, formulated in 0.1 M Tris-Glycine buffer (pH 7.4) with 150 mM NaCl, 0.2 % sodium azide and 50 % glycerol as a preservative, and should be stored according to standard short-term conditions of -20 °C to preserve activity. The host species is Mouse, the clonality is monoclonal, and the immunogen corresponds to purified recombinant human AMPKβ1 protein fragments expressed in E. coli, which gives the clone strong selectivity for endogenous AMPKβ1 with negligible cross-reactivity to related proteins. The antibody has been validated for Western blotting (recommended at 1:1000 dilution, expected band ~38 kDa), Immunocytochemistry (ICC), Immunohistochemistry (IHC) and Immunoprecipitation (IP), making it suitable for both denaturing lysate work and native complex pull-downs. Reactivity has been confirmed in Human, Mouse, Rat and non-human primate (Monkey/Simian) samples, with supporting validation data reported in 3T3, HeLa, PC-12, COS7 and MDA-MB-468 cell lysates on the Antibodies.com product page. As AMPKβ1 forms part of the heterotrimeric AMPK complex that senses cellular energy status via AMP:ATP ratios, this reagent is widely used in metabolism, diabetes, cancer and exercise-physiology research, and pairs well with companion anti-AMPKα and anti-AMPKγ antibodies for subunit-resolved studies of AMPK signaling.