Anti-Amphiphysiniphysin isoform 1 Antibody

Raised in rabbit and supplied as an unconjugated polyclonal antiserum, this reagent from Antibodies.com targets amphiphysin isoform 1, a neuronal BAR- and SH3-domain protein best known for its role in clathrin-mediated synaptic vesicle endocytosis. The antibody is reactive with human samples and is validated for western blot and immunohistochemistry, making it a practical tool for neuroscience and paraneoplastic disease research, where amphiphysin is used as a marker of neuronal tissue and of the autoantibody response seen in stiff-person syndrome associated with breast carcinoma. Each vial contains 100 µl of reagent; for repeated use, aliquot the antiserum and store frozen, protecting the vial from repeated freeze-thaw cycles to preserve antibody activity. Because amphiphysin 1 concentrates at nerve terminals and at the leading edge of non-neuronal cells undergoing endocytosis, researchers commonly apply this reagent to localize synaptic active zones in human brain sections and to confirm expression of the ~128 kDa neuronal isoform by immunoblotting of neuronal lysates. The polyclonal format improves the chance of recognizing the target even when epitope accessibility varies across fixation conditions.