Anti-AmCyan Antibody

Raised in rabbit, this unconjugated polyclonal reagent from Antibodies.com binds the cyan fluorescent protein variant AmCyan, which originates from the sea anemone Anemonia majano and is widely deployed as a genetically encoded fusion tag in molecular and cell biology. Researchers use the antibody to detect AmCyan-tagged constructs across western blot, immunohistochemistry on fixed tissue sections, immunofluorescence imaging of fixed cells, and immunoprecipitation of native complexes from cell lysates, supporting workflows that range from verifying reporter expression to pulling down tagged proteins for downstream interaction studies.

Supplied as 100 µL of antiserum, the reagent should be stored refrigerated at 4 °C to preserve activity, with working aliquots kept on ice and protected from light to limit photobleaching of any residual fluorophore. The polyclonal format targets multiple epitopes on the roughly 27 kDa AmCyan monomer, which can improve signal recovery in western blot and IP applications where the tag is folded into a fusion partner. As an unconjugated primary antibody, the product is compatible with user-selected reporter-coupled secondary antibodies and standard chromogenic or fluorescence detection chemistries, making it adaptable to multicolor panel design where AmCyan co-expression must be confirmed alongside other lineage or localization markers.