Anti-ALPP Antibody

ALPP encodes placental alkaline phosphatase, a GPI-anchored membrane ectoenzyme that is normally restricted to placental trophoblasts but is re-expressed in seminomas, ovarian carcinomas, and other germ-cell tumors, where it functions as both a diagnostic marker and a model substrate for studying glycosylphosphatidylinositol-anchored protein trafficking. Antibodies.com raises this rabbit polyclonal IgG reagent against a region of the human ALPP protein, and the resulting antiserum is supplied as 100 µL of unconjugated primary antibody in a single vial ready for dilution into standard blocking buffer.

Validation data from Antibodies.com confirms reactivity on human and rat samples, with clean signals in western blot of placenta-derived lysates and efficient immunoprecipitation of the native enzyme from membrane-enriched fractions. The unconjugated IgG format lets laboratories pair the reagent with their own HRP, biotin, or fluorophore-conjugated secondaries, and the polyclonal profile broadens recognition of ALPP isoforms carrying distinct glycosylation states. Researchers investigating trophoblast differentiation, germ-cell tumor biology, or alkaline phosphatase enzymology can integrate this antibody into immunoblotting, IP, and downstream phosphatase activity workflows without cross-species optimization.

For best results, briefly centrifuge the vial before opening, aliquot the 100 µL volume into single-use portions to limit freeze-thaw cycles, and store refrigerated between uses; long-term storage should be at -20 °C or colder in a frost-free freezer with desiccation to preserve IgG activity over months of repeated experiments.