Anti-alpha Glucosidase II Antibody

Raised against the catalytic alpha subunit of endoplasmic reticulum glucosidase II, encoded by GANAB, this unconjugated rabbit polyclonal reagent from Antibodies.com recognizes the ER-resident heterodimeric enzyme that trims the middle and innermost alpha-1,3-linked glucose residues from Glc2Man9GlcNAc2 N-glycans on nascent secretory glycoproteins during the calnexin and calreticulin chaperone cycle. The 100 µl liquid vial is formatted for western blot, immunohistochemistry, and immunofluorescence on human samples and cultured cells.

Because the antibody is unconjugated, it can be paired with HRP, biotin, Alexa Fluor, or other secondary reporters depending on the assay, giving laboratories a flexible primary reagent for studies of N-glycan processing, ER-associated degradation, secretory pathway flux, and disorders linked to GANAB sequence variation. The polyclonal format provides multi-epitope recognition, which helps when probing denatured lysates by western blot or fixed tissue sections by IHC where a single monoclonal may drop signal at a masked epitope.

For handling, refrigerate the vial at 4 °C for short-term use and aliquot before freezing for long-term storage; repeated freeze-thaw cycles reduce polyclonal antibody titer over time. Each shipment from Antibodies.com includes lot documentation covering host species, clonality, target, and reactivity so investigators can record provenance in their methods sections.