Anti-alpha Actinin 4 Antibody

This unconjugated rabbit polyclonal antibody from Antibodies.com binds alpha-actinin 4, an actin-bundling protein of the spectrin superfamily that anchors actin filaments at focal adhesions, cell-cell junctions, and the Z-discs of striated muscle. The reagent is supplied as 100 µl in a vial, ready for direct dilution into standard immunodetection buffers.

Alpha-actinin 4 is best known for its role in podocyte cytoskeletal integrity, where dominant mutations in ACTN4 produce a familial form of focal segmental glomerulosclerosis. Beyond renal biology, the protein is implicated in tumor cell motility, with elevated levels reported in invasive carcinomas and correlated with metastatic potential. The polyclonal format provides broad epitope recognition, useful for detecting both full-length ACTN4 and splice or post-translationally modified variants.

Validated applications include western blot, immunohistochemistry, and immunofluorescence on human specimens. For working stocks, aliquot the reagent upon arrival and avoid repeated freeze-thaw cycles; store refrigerated for short-term use and frozen for longer-term retention.