Anti-alpha Actin Antibody [M8]

This Mouse monoclonal IgG1 antibody (clone M8) from Antibodies.com specifically recognizes alpha actin, a cytoskeletal protein abundantly expressed in smooth muscle cells and myofibroblasts. With reactivity across human, mouse, and rat samples, the unconjugated reagent is supplied as a 100 µg liquid formulation in a single vial, ready for use in western blot, immunocytochemistry, and immunohistochemistry on paraffin-embedded tissue sections. The antibody detects the target band at the expected molecular weight of approximately 42 kDa, making it a reliable marker for studies of smooth muscle differentiation, fibrosis, and vascular remodeling. For long-term stability, aliquot the antibody and store at -20°C, avoiding repeated freeze-thaw cycles. Recommended starting dilutions are 1:500 to 1:2000 for western blot and 1:100 to 1:500 for IHC-P, though optimal conditions should be determined empirically per assay.