Anti-alpha 1 Antichymotrypsin Antibody [B4H12]

The B4H12 monoclonal antibody from Antibodies.com is a mouse IgG1 reagent raised against alpha 1 Antichymotrypsin (SERPINA3), a liver-derived acute-phase serine protease inhibitor implicated in inflammatory responses and in the protein deposition observed within Alzheimer's disease amyloid plaques. Supplied as a lyophilised 100 µg vial, the unconjugated clone supports reproducible detection of the human target in standard immunoblotting workflows.

Validated for western blot, the antibody recognizes human alpha 1 Antichymotrypsin and can be paired with an anti-mouse IgG secondary reagent for chemiluminescent signal development. Reconstitution in sterile distilled water yields a working stock suitable for SDS-PAGE and membrane transfer; small aliquots are recommended to limit repeated freeze-thaw cycles, and the reconstituted preparation should be stored at -20 °C for routine use.

Researchers investigating serpin biology, hepatic acute-phase signaling, neurodegeneration-associated protein aggregation, and tumor-associated inflammation may use this clone to confirm SERPINA3 expression in cell lysates and tissue extracts. The mouse host species and IgG1 isotype are documented in the product specification, which also simplifies selection of isotype-matched controls when designing multiplexed immunoblotting panels or comparing antibody pairs across related targets in the serpin family.