Anti-ALKBH5 Antibody

Anti-ALKBH5 polyclonal antibody for m6A demethylase research

This rabbit polyclonal IgG antibody from Antibodies.com targets ALKBH5 (AlkB homolog 5), a nuclear Fe(II) and 2-oxoglutarate-dependent dioxygenase that catalyzes oxidative demethylation of N6-methyladenosine (m6A) on messenger RNA. The reagent is supplied unconjugated in a 200 µL vial and is validated for western blot detection of the endogenous human protein in cell and tissue lysate workflows.

Raised in rabbit and purified as a polyclonal IgG preparation, the antibody is compatible with standard anti-rabbit IgG secondary reagents for chemiluminescent western blot readouts. Researchers studying post-transcriptional RNA modification, mRNA nuclear export, hypoxia response, spermatogenesis, and tumor biology use ALKBH5-targeting antibodies to investigate demethylase expression in cancer models and crosstalk with other m6A regulators such as METTL3, FTO, and YTHDF2.

Recommended starting protocol: resolve whole-cell or nuclear extracts by SDS-PAGE, transfer to a suitable membrane, block, and probe with the primary antibody followed by an HRP- or fluorophore-conjugated anti-rabbit IgG secondary. Aliquot upon receipt to limit freeze-thaw cycles; store refrigerated short-term at 4 °C and frozen long-term at -20 °C in small working volumes to preserve antibody activity.