Anti-ALKBH5 Antibody

Produced in rabbit as an unconjugated polyclonal IgG and validated for Western blot detection of the human target, this ALKBH5 reagent from Antibodies.com is supplied at a 100 µl working volume for studies of post-transcriptional RNA modification, m6A epitranscriptomic regulation, and expression dynamics in cell-line and tissue lysates.

ALKBH5 (alkB homolog 5) is a nuclear Fe(II) and 2-oxoglutarate-dependent RNA demethylase that catalyzes oxidative removal of N6-methyladenosine (m6A) marks from transcribed RNAs and is studied alongside the writer complex METTL3/METTL14, the related demethylase FTO, and the YTHDF family of m6A reader proteins. Research links ALKBH5 activity to spermatogenesis, stress-granule assembly, hypoxia-responsive gene expression, and tumor progression, so reliable immunodetection is frequently required to confirm CRISPR or RNAi knockdown efficiency, to compare expression across treatment conditions, and to normalize transcript-level m6A measurements against protein abundance on immunoblots.

The unconjugated format of this ALKBH5 antibody permits pairing with user-preferred HRP, fluorescent, or biotinylated secondary reagents, supporting chemiluminescent, near-infrared, or fluorescent Western blot workflows. Reactivity is established for human protein, while use on non-human samples should be supported by independent empirical validation, since broader species cross-reactivity has not been characterized for this specific reagent.