Anti-ALKBH3 Antibody

ALKBH3 is an Fe(II)/2-oxoglutarate-dependent dioxygenase that repairs alkylation damage in single-stranded DNA and RNA, including 1-methyladenine and 3-methylcytosine lesions. This rabbit polyclonal antibody from Antibodies.com is raised against ALKBH3 and validated for Western blot detection across human, mouse, and rat samples. The unconjugated IgG preparation is supplied at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol as a cryoprotectant, shipped as a 100 µl aliquot. Polyclonal reactivity against multiple epitopes on the demethylase enables detection of full-length ALKBH3 in cell lysates and tissue extracts; investigators probe whole-cell lysates prepared in RIPA buffer, resolving proteins by SDS-PAGE before transfer to nitrocellulose or PVDF membranes. Storage at -20°C in single-use aliquots limits freeze-thaw cycles and preserves antibody activity. The reagent serves research into direct DNA damage reversal, alkylation resistance phenotypes, and the role of ALKBH3 in tRNA and mRNA demethylation pathways relevant to cancer cell stress responses.