Anti-Aldose Reductase Antibody

Aldose reductase is a 36 kDa cytosolic monomer belonging to the aldo-keto reductase superfamily that catalyzes the NADPH-dependent reduction of glucose to sorbitol, the first and rate-limiting step of the polyol pathway. Overactivity of this enzyme in hyperglycemic conditions drives intracellular accumulation of sorbitol and contributes to diabetic complications including retinopathy, nephropathy, and neuropathy, making it a long-standing target for diabetes research and small-molecule inhibitor screening. The rabbit polyclonal antibody supplied by Antibodies.com at 100 µL per vial was raised against aldose reductase and validated for Western blot, immunohistochemistry, immunofluorescence, and ELISA workflows. The unconjugated reagent specifically recognizes the human and rat orthologs, supporting comparative studies across species that model diabetic tissue damage in vivo and in cell culture systems. For laboratory planning, prepare a working aliquot from the stock vial to limit freeze-thaw cycles, and store the antibody frozen at -20 °C in single-use aliquots to preserve antigen-binding activity over time. Researchers investigating polyol pathway flux, mechanisms of diabetic complications, or the pharmacology of aldose reductase inhibitors can deploy this reagent across cultured cell lysates, tissue sections, and recombinant protein samples.