Anti-ALDH1A2 Antibody

Produced in rabbit and affinity-purified from rabbit antiserum, this 200 µl unconjugated polyclonal IgG from Antibodies.com detects ALDH1A2 (also known as RALDH2 or retinal dehydrogenase 2), the cytosolic retinaldehyde dehydrogenase that catalyzes the oxidation of retinaldehyde to retinoic acid during vertebrate embryogenesis. Because localized retinoic acid synthesis patterns the posterior hindbrain, drives cardiac looping and outflow tract morphogenesis, and guides spinal motor neuron specification, the reagent is widely used to map active RA signaling in fixed tissues and lysates from human, mouse, and rat samples. Validation for Western blot resolves the approximately 57 kDa ALDH1A2 band on membranes, while immunohistochemistry on paraffin or cryosections reveals regional expression in the sinus venosus, somites, testis, and other retinoid-dependent tissues. The liquid formulation in phosphate-buffered saline with 50% glycerol and 0.02% sodium azide should be stored at -20 °C in single-use aliquots to limit freeze-thaw damage. Developmental biology, stem cell biology, and oncology groups — where ALDH1A2 status is monitored as a differentiation marker and has been linked to chemoresistance in ovarian carcinoma, mesothelioma, and head and neck squamous cell tumors — rely on the same vial for both exploratory runs and larger cohort screens.