Anti-ALAS2 Antibody

The unconjugated rabbit polyclonal antibody from Antibodies.com targets ALAS2 (5'-aminolevulinate synthase 2), the erythroid-specific mitochondrial enzyme that catalyzes the first and rate-limiting step of heme biosynthesis in developing red blood cells. Affinity-purified and supplied as 100 µl of liquid stock, this polyclonal reagent is validated for Western blot (WB) and immunofluorescence (IF) applications against human samples. ALAS2 mutations underlie X-linked sideroblastic anemia, and altered expression features in erythropoietic porphyrias, making this antibody useful for studies of erythroid differentiation, mitochondrial iron handling, and heme pathway regulation. The unconjugated format allows flexible pairing with secondary antibodies labeled with HRP, Alexa Fluor, biotin, or other reporters for chromogenic or fluorescence detection. Researchers commonly apply IF on fixed cell preparations and WB on whole-cell lysates from erythroid cell lines such as K562 or HEL. Polyclonal recognition spans multiple epitopes, which is helpful when target abundance is low or when a single epitope is masked by sample preparation. For long-term storage, aliquot the vial and freeze at -20 degrees C; working stocks remain stable at refrigerated temperatures for several weeks. Protect from light and minimize freeze-thaw cycles to preserve activity over the product's shelf life. Each 100 µl vial is intended for research use only and supports multiple replicate experiments across WB and IF workflows in Human-derived samples.