Anti-Akt3 Antibody [K17-H]

This primary antibody from Antibodies.com is a rabbit monoclonal reagent (clone K17-H) raised against Akt3, a serine/threonine kinase of the AKT/PKB family implicated in neuronal survival, metabolic regulation, and growth-factor-driven signaling in human cells. Conjugated to FITC, the reagent is supplied as a ready-to-use liquid in a 100-test vial and is validated for flow cytometry, where the fluorescein label is excited by the standard 488 nm blue laser and emits in the green channel near 519 nm. The IgG isotype framework allows direct staining without a secondary reagent, and the liquid format eliminates the need for reconstitution prior to use. For integrity, store the vial refrigerated and protected from light; repeated freeze-thaw cycles should be avoided because the FITC fluorophore and the protein conjugate are both sensitive to such stress. Researchers characterizing kinase signaling cascades, neuronal Akt3 expression, or downstream phosphorylation events in tumor-derived human cell lines can apply this clone to surface and intracellular Akt3 detection by flow cytometry, taking advantage of the FITC label for clear separation in the standard green detection channel.