Anti-Akt2 (phospho Ser478) Antibody [S7-R]

Phospho-Akt2 flow-cytometry reagent: This rabbit monoclonal primary antibody from Antibodies.com detects Akt2 phosphorylated at Ser478 in human samples. The liquid reagent is directly conjugated to FITC, allowing fluorescent Akt2 analysis without a labeled secondary antibody. It is supplied in a 100T size and has an IgG isotype.

Use the antibody to examine Akt2 phosphorylation across human cell populations, compare signaling responses after stimulation or pathway inhibition, and identify heterogeneous phospho-protein states. Its FITC label supports incorporation into multicolor flow-cytometry panels when paired with antibodies detected in compatible channels. The S7-R clone provides a consistent monoclonal reagent for studies focused on the phosphorylated Akt2 target rather than total Akt2 abundance.

For reliable results, include appropriate unstained, single-color, and phospho-positive or phospho-negative controls. Because phosphorylation-dependent epitopes can be affected by sample handling, fixation, and permeabilization, staining conditions should be optimized and validated for the specific cell type and experimental treatment.