Anti-AKT2 (phospho-S474) Antibody

Raised in rabbit to selectively recognise AKT2 phosphorylated at serine 474, this unconjugated polyclonal antibody from Antibodies.com distinguishes the activated form of RAC-beta serine/threonine-protein kinase from the inactive, unphosphorylated pool. Phosphorylation at this activation-loop residue is the molecular switch that confers full catalytic activity within the PI3K/AKT signalling cascade, making the reagent a targeted probe for pathway activation studies in oncology, metabolism, and insulin signalling research.

Validated applications include Western blot on cell and tissue lysates and immunohistochemistry on formalin-fixed paraffin-embedded sections. The unconjugated IgG preparation supports chromogenic and fluorescence imaging workflows, or downstream labelling with user-supplied secondary reagents. Cross-reactivity has been confirmed with human, mouse, and rat samples.

Provided as 100 µl at 1 mg/ml in phosphate buffered saline with 0.05% sodium azide at approximately pH 7.2. Refrigerate on receipt to preserve antibody activity; the sodium azide serves as a microbial preservative. Handle small aliquots with clean tips to avoid repeated freeze-thaw cycles of the working stock.